نشریه پژوهش های بالینی دام های بزرگ
پیاپی 19 (پاییز 1392)

Antioxidant and Antiapoptotic Effect of Resveratrol in Streptozotocin Induced Diabetic Rats. Oxidative stress is strongly associated with development and the complications of diabetes. In the present study¡ we have investigated the effect of resveratrol on antioxidants status in streptozotocin induced diabetic rats.48 male wistar rats with were randomly assigned into four equal groups namely healthy control vehicle treated group (normal saline)¡ healthy group received resveratrol (5 mg/kg)¡ diabetic control group and diabetic group treated with resveratrol (5 mg/kg). The experimental rats were treated in related groups for 16 weeks and at the end of experiment the level of malonaldehyde (MDA) and anti-oxidant enzymes activity including superoxide dismutase (SOD)¡ glutathione peroxidase (GPx) and catalase (CAT) were measured in red blood cells. Significant differences among the groups were determined by one-way analysis of variance followed by Bonferroni post-test. Statistical significance was considered (p

The main goals of treatment in the mandibular fractures include bone union¡ normal dental occlusion and immediate return to oral alimentation. In this study twelve adult cats were divided into two groups. A segmental defect of 5mm was created in one of the hemimandibles behind the 1st molar¡ the fractured site was fixed in both group using mini bone plate with 4 screws¡ then demineralized bone matrix (DBM) was used to fill the gap after fracture fixation in Group II. Radiography was done after creating defects on 0¡ 15¡ 30 and 60 days¡ from the radiological results the parameters of groups were compared with Lane and Sandhu scale system in terms of callus tissue¡ mineralization and remodeling ¡ the statistical data showed that group 2 differ significantly from the other group in terms of total scoring (p

In order to study of the khouzestanian water buffalo karyotype from whole blood of 200 Animals has been collected then 7ml of whole blood was cultured in RPMI1640 medium culture and phytohemagglutinin type A (PHA-A) was used as mitogen agent and incubated at 37ºC for 72 hours. Cell division was stopped in metaphase with adding 200 µl of colchicine to media after 72 hours. White cells has been driven by Sumner´s method¡ then chromosomes was spread on frizzed lams. Sumner´s methods were used for staining and banding of chromosomes. Cell spreads were fixed with heat and Entellan Merck. Centromere index and partial length of chromosomes were determined from photo micrographs with 200x enlargement. The results showed that all breeds have a diploid chromosome number of 2n=50. Each karyotype consisted of 24 pairs of autosomes ¡that 5 pairs of them were metacentric and 19 pairs of them were acrocentric. Chromosome number 1 with6.72±0.0178 partial length was the largest chromosome. X chromosome with6.25±0.056 partial length was the largest acrocentric chromosome and the Y chromosome with 1.872±0.016 was the smallest chromosome acrocentric.

Mastitis causes great economic losses including reduction of milk production and quality as well as milk microbial contamination is considered as a means of disease transmission to humans. Therefore investigating various aspects of the disease can be effective in controling and decreasing damages due to the disease. In this study¡ 200 samples of raw milk from cows with clinical mastitis or cows which showed different degree of positivity in the California mastitis test were tested. After colony identification¡ antibiotic susceptibility test was performed by disk diffusion method. Cultures were positive in 152 cases (%76) and negative in 48 cases (%24). Highest frequency belonged to species Micrococcus luteus (%39.5)¡ Micrococcus varians (%14.5)¡ Staphylococcus epidermidis (%10.5)¡ E.Coli (%6.6)¡ Staphylococcus aureus (%2.6) and lowest frequency belonged to the Serratia (%1.3). In the antibiogram test the most sensitivity of agents genus was to contamination was Gentamycin (%80.31)¡ Enrofloxacin (%77)¡ Ciprofloxacin (%66) and Oxytetracyclin (%48) and the least sensitive was to Cefixime (%9.9). Results derived from the antibiogram tests in the study indicates the necessity of similar periodic studies which can be considered a regional therapeutic programme.

This survey was carried out in a 7 months period from September 2011 to March 2012¡ in slaughter house of yasuj city¡ the center of Kohgilouyeh and Boyerahmad Province. At this study 13206 sheep kidneys and 21350 goat kidneys were examined for presence of renal lesions macroscopically. Relative abundance of kidney condemnation in sheep and goats was determined 0.8% (106/13206) and 1.2% (265/21350) respectively. For histopathologic study samples from 97 condemned kidneys of sheep and 94 condemned kidneys of goats were collected in 10% buffered formalin and sent to the laboratory of pathology. According to histopathologic findings¡ Interstitial Nephritis as the most abundant lesion was observed in 69 cases (71.13%) of sheep kidneys and 68 cases (72.34%) of goat kidneys. The other renal lesions observed in sheep were purulent abscess in 15 cases (15.47%)¡ pyelonephritis in 6 cases (6.18%)¡ nephrosis in 5 cases (5.16%)¡ hydatid cyst in 2 cases (2.06%). In goats the other renal lesions were purulent abscess in 10 cases (10.64%)¡ pyelonephritis in 4 cases (4.25%)¡ nephrosis in 4 cases (4.25%)¡ Amyloidosis in 2 cases (2.13%)¡ hydatid cyst in 3 cases (3.19%)¡ infarction in 2 cases (2.25%) and Congestion in one case (1.07%). Statistical analysis showed significance differences (P

Kazeroon in the southwest city of the Fars province is the second regional Iran` climate.. The Present investigation was performed to order to determine the diversity and intensity of ticks sheep''s in kazeroon city.For the study¡ the sheep''s from central region and Khesht¡ Konartakhte¡ Baladeh¡ Chenashahigan¡ Kohmare and Nodan regions during October 2008 to September 2009¡ where examined in different parts of the body. Overall 2300 evaluated sheep''s¡ 227 sheep''s (9.86 %) were infested to tick in witch 881 ticks were collected. From this amount of ticks 394 ticks were male (44.8 %) and 487 ticks were female (55.2 %).The identified species were as follows: Rhipicephalus bursa (59 %)¡ R.sanguianus (13 %)¡ Hyaloma asiaticum asiaticum (18%)¡ H. anatolicum anatolicum (6 %)¡ Dermacentor marginatus (4 %). Distribution of ticks over different part of sheep body surface was respectively as follow¡ according to its frequencies: tail region¡ (41%)¡ inguinal region¡ (23 %)¡ head and neck¡ (20 %)¡ breast (14 %) and testes (2%). The prevalence and intensity of infection was low but high species diversity was seen.

Finding a human based antigen is either time consuming¡ hazardous¡ expensive or very difficult and if applicable it is in very low scale. Therefore the aim of the current study was to compare the human responses against antigen B¡ cow¡ sheep and mice crude hydatid cyst fluid (HCF) antigens of Echinococcus granulosus. Hydatid cysts obtained from lungs and livers of sheep and cattle slauthered from local abattoirs in Ilam city¡ and was used as the source of antigen either for the SDS¡ Western blotting or ELISA analysis or for experimentally infecting the mice. 30 samples of sheep and 30 samples of cattle hydatid cyst fluid antigen were used as the source of antigen and also to experrimentally infect the mice. human sera samples were collectesd from surgery section of hospitals in Ilam city . IgG and IgE responses were measured using ELISA in case-control study design. Each response was comparly to the others. Results were analysed using Anova and Post Hoc methods. HCF recovered from cysts was centrifuged at 10000 rpm for 5 minutes. The supernatant was dialysed against PBS¡ freezed-thawed and used as crude HCF.There was an increasing in IgG4 subclass level against cattle hydatid cyst crude antigen with 10.2 times against cut off and B antigen with 9 times against cut off. In this analysis all IgG class and subclasses and IgE antibody responses showed a higher mean OD than that of the cut off¡ indicating that all human sera from patients with hydatidosis reacted againstcow¡ sheep and mice crude hydatid cyst fluid antigens and also antigen B. There was an acceptable cross reaction of human immune responses against cattle¡ sheep¡ mice and B with that of human using ELISA. Mice antigen is suitable for designing a diagnostic kit. Human total IgG and IgG4 had the highest responses against these antigens comparing to other antibodies.Hyadatid cyst fluid antigen of both cattle and sheep were significantly useful in serodiagnosis of hydatidosis similar to that of human and therefore can be used in hydatid cyst diagnosis kit using ELISA.

Apoptosis is a form of cell death that is often regular and targeted in some cells¡ but it can be induced whit some drugs and toxins or pathogens also.This research was carried out on 240 one day-old male broiler chickens (Ross 308) in a completely randomized design (CRD) in four treatments at 0(control)¡ 4 ¡ 8 and 12 ppm levels of silver nanoparticles that was added to water intake with four repetitions within 16 separate cages and 15 birds in each pen. At the end of research (day 42)16 chicks were randomly selected and slaughtered and their kidney were examined with light microscope. TUNEL stainingshowed significant (p

In this research¡ effects of diet containing 0.5¡ 1 and 1.5 percent aminabad Qom zeolite and 1 percent commercial zeolite(anzemite) investigated on microbial load of intestine broilers. 400 day old male broiler chicks were bought and were allocated to 20 pens(5 treatments with 4 repetitions) equally. The control treatment got basal diet only. All chicks had kept in the same condition. In 42 day¡ 2 chicks to be butchered from each pen and sampling was done from the intestine of them. The samples were cultured in BA and EMB by serial dilution method .Colonies was accented after 24 h in 37˚. Measured PH of intestinal samples . E.coli were isolated from intestinal samples. They were tested antibiogram. The results showed that supplementation with zeolite don''t make a change in ph compared with the control diet¡ and that chicks fed with zeolite didn''t showed significant change in total microbial load . Supplementation with zeolite significantly reduced the counts of Entrobacteriaceae in the intestine(p

This experiment was conducted to assess the effect of fat and Monensin in diet on daily dry matter intake¡ total feed intake¡ final weight¡ total weight gain¡ daily weight gain¡ feed conversion ratio¡ blood metabolites (glucose¡ urea¡ calcium¡ phosphorous¡ triglycerides¡ cholesterol and total protein) in Mazandaran Zel lambs breed. A factorial design of 2×3 was used in this experiment (two levels of fat¡ 0 & 4 percent and three levels of Monensin¡ 0¡ 20 & 40 mg per kg respectively). twenty four male lamb from Mazandaran Zel breed with average weight of 21.9±0.64 kg and 3-4 months ages were used. The experiment was conducted with an adaptation period of eleven days and the experimental period lasted for 63 days. Lambs live body weight was determined in a 21 days interval. blood samples were drived from each experimental lambs at the end of experiment were analysed to determine the mentioned metabolites. The results of this experiment indicated that the effect of different levels of fat & monensin on daily dry matter intake¡ total feed intake¡ final weight¡ total weight gain¡ daily weight gain¡ feed conversion ratio & blood glucose was not significant¡ but a significant effect was observed (p